PRR7 antibody

• Catalog Number: orb44753
• Category: Antibodies
• Description: Mouse Monoclonal to PRR7.
• Clonality: Monoclonal
• Clone Number: TRAP3/10
• Tested applications: ICC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG2a
• Immunogen: Recombinant C-terminal half of the intracellular domain of human PRR7/TRAP3 (amino acids 126-253)
• Concentration: 1 mg/ml
• Dilution range: Immunocytochemistry: Recommended dilution: 10 μg/ml; cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton-X100. Western blotting: Recommended dilution: 1 μg/ml; positive control: murine brain lysate (red. Laemmli buffer).
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: PRR7
• Entrez: 80758
• UniProt ID: Q8TB68
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: PRR7 antibody, anti-TRAP3
• Note: For research use only
• Application notes: Immunocytochemistry: Recommended dilution: 10 μg/ml; cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton-X100. Western blotting: Recommended dilution: 1 μg/ml; positive control: murine brain lysate (red. Laemmli buffer).
• Expiration Date: 12 months from date of receipt.

Western blotting detection of PRR7 / TRAP3 in murine brain lysate using the monoclonal antibody TRAP3/10.

Immunocytochemistry staining of HEK-293 cells cotransfected with PRR7 / TRAP3 (red) and GFP-PSD-95 (green). PRR7 / TRAP3 detected by monoclonal antibody TRAP3/10. DNA visualized by DAPI (blue).

Western blotting analysis of PRR7 using mouse monoclonal antibody TRAP3/10 on rat neuron lysate, and on HeLa transfectants overexpressing human PRR7, compared with non-transfected HeLa cells (negative control). Nitrocellulose membrane was probed with 1 µg/ml of mouse anti-PRR7 followed by a secondary antibody. PRR7 was detected around 35 kDa.