PRPH Antibody

• Catalog Number: orb1257772
• Category: Antibodies
• Description: PRPH Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PRPH (NP_006253.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:20 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 54-62kDa
• Target: PRPH
• UniProt ID: P41219
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: NEF41, PRPH, peripherin, neurofilament 4 (57kD)
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:20 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PRPH antibody (orb1257772) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat heart using PRPH antibody (orb1257772) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using PRPH antibody (orb1257772) at dilution of 1:100 (40x lens).