Proxtract B Extraction Buffer

SKU: orb348596

Description

Proxtract B Extraction Buffer

Key Properties

• Purity: ProXtract B Extraction Buffer was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store container at room temperature (18° to 26° C) prior to opening. If desired, the solution may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) to restore solubility of some salts.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.05% (w/v) Sodium Azide
• Concentration: 1X
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

ProXtract B Extraction Buffer, Protein Extraction Buffer, Bacterial Protein Extraction Reagent, Bacterial Cell Lysis Buffer

SDS-PAGE of Construct A, B and C plasmids transformed in E.Coli BL21 strains. After overexpression, cells were pelleted by centrifugation at 5000 rpm for 10 mins. Protein extraction was performed with ProXtract buffer for 20 mins at RT. Centrifugation is then done at 12000 rpm for 20 minutes at 4⁰C. Lane 1: Supernatant of Construct A. Lane 2: Pellet from Construct A. Lane 3: Supernatant of Construct B. Lane 4: Pellet from Construct B. Lane 5: Supernatant of Construct C. Lane 6: Pellet from Construct C.

SDS-PAGE of recombinant protein D/ pGEX-2T plasmid transformed in Rosetta strain. Cells were plated on LB Cam-Amp, grown, and induced with IPTG. Cells were pelleted by centrifugation at 5000 rpm for 10 mins. Protein extraction was performed with ProXtract buffer for 20 mins at RT. Centrifugation is then done at 12000 rpm for 20 minutes at 4⁰C. Lane 1: Pellet from 1h. Lane 2: Supernatant of 1h. Lane 3: Pellet from 2h. Lane 4: Supernatant of 2h. Lane 5: Pellet from 3h. Lane 6: Supernatant of 3h.