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Proxtract B Extraction Buffer
Catalog Number: orb348595
| Catalog Number | orb348595 |
|---|---|
| Category | Tools |
| Description | Proxtract B Extraction Buffer |
| Conjugation | Unconjugated |
| Form/Appearance | Liquid (sterile filtered) |
| Concentration | 1X |
| Buffer/Preservatives | 0.05% (w/v) Sodium Azide |
| Purity | ProXtract B Extraction Buffer was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria. |
| Tested applications | WB |
| Dilution range | WB: 2-5µL |
| Application notes | ProXtract B Buffer is suitable for protein extraction and easily scalable. Specific conditions should be optimized by the end user. See protocol. |
| Storage | Store container at room temperature (18° to 26° C) prior to opening. If desired, the solution may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) to restore solubility of some salts. |
| Alternative names | ProXtract B Extraction Buffer, Protein Extraction Read more... |
| Hazard Information | Non-Toxic |
| Note | For research use only |
SDS-PAGE of Construct A, B and C plasmids transformed in E.Coli BL21 strains. After overexpression, cells were pelleted by centrifugation at 5000 rpm for 10 mins. Protein extraction was performed with ProXtract buffer for 20 mins at RT. Centrifugation is then done at 12000 rpm for 20 minutes at 4⁰C. Lane 1: Supernatant of Construct A. Lane 2: Pellet from Construct A. Lane 3: Supernatant of Construct B. Lane 4: Pellet from Construct B. Lane 5: Supernatant of Construct C. Lane 6: Pellet from Construct C.
SDS-PAGE of recombinant protein D/ pGEX-2T plasmid transformed in Rosetta strain. Cells were plated on LB Cam-Amp, grown, and induced with IPTG. Cells were pelleted by centrifugation at 5000 rpm for 10 mins. Protein extraction was performed with ProXtract buffer for 20 mins at RT. Centrifugation is then done at 12000 rpm for 20 minutes at 4⁰C. Lane 1: Pellet from 1h. Lane 2: Supernatant of 1h. Lane 3: Pellet from 2h. Lane 4: Supernatant of 2h. Lane 5: Pellet from 3h. Lane 6: Supernatant of 3h.
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