Prothrombin/F2 Antibody

• Catalog Number: orb402178
• Category: Antibodies
• Description: Prothrombin/F2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human Prothrombin recombinant protein (Position: Y67-R124).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 85 kDa
• UniProt ID: P00734
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Prothrombin; Coagulation factor II; Activation pep
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of H-PBMC cells using anti-Prothrombin antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Prothrombin using anti-Prothrombin antibody.Lane 1:human plasma lysate.

IHC analysis of Prothrombin using anti-Prothrombin antibody.Prothrombin was detected in paraffin-embedded section of human colon cancer tissue.

IHC analysis of Prothrombin using anti-Prothrombin antibody.Prothrombin was detected in paraffin-embedded section of human lung cancer tissue.