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Catalog Number | orb1248285 |
---|---|
Category | Antibodies |
Description | proteinase 3/myeloblastin Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-HNVRTQEPTQQ |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 28kDa band observed in Human Spleen lysates (calculated MW of 27.8kDa according to NP_002768.3). Recommended concentration:0.3-1ug/ml. Primary incubation was 1 hour |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Approx 27kDa |
Target | proteinase 3 / myeloblastin |
UniProt ID | P24158 |
NCBI | NP_002768.3 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | PRTN3, proteinase 3, ACPA, AGP7, C-ANCA, CANCA, MB Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 28kDa band observed in Human Spleen lysates (calculated MW of 27.8kDa according to NP_002768.3). Recommended concentration:0.3-1ug/ml. Primary incubation was 1 hour |
Expiration Date | 12 months from date of receipt. |
orb1248285 (0.3 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1248285 (5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
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