Protein Kinase A regulatory subunit I alpha/PRKAR1A Antibody

• Catalog Number: orb389508
• Category: Antibodies
• Description: Protein Kinase A regulatory subunit I alpha/PRKAR1A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human PRKAR1A recombinant protein (Position: E2-E81). Human PRKAR1A shares 89.9% and 92.4% amino acid (aa) sequence identity with mouse and rat PRKAR1A, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Western blot, 0.1-0.5μg/ml, Human, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 42982 MW
• UniProt ID: P10644
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: cAMP-dependent protein kinase type I-alpha regulat
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of PRKAR1A using anti-PRKAR1A antibody.Lane 1:rat thymus tissue;2:human HepG2 cell;3:human MCF-7 cell.

IHC analysis of PRKAR1A using anti-PRKAR1A antibody. PRKAR1A was detected in a paraffin-embedded section of rat testis tissue.

IHC analysis of PRKAR1A using anti-PRKAR1A antibody. PRKAR1A was detected in a paraffin-embedded section of human lung cancer tissue.

IHC analysis of PRKAR1A using anti-PRKAR1A antibody. PRKAR1A was detected in a paraffin-embedded section of human intestinal cancer tissue.