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Catalog Number | orb348760 |
---|---|
Category | Proteins |
Description | Protein G Peroxidase Conjugated |
Tested applications | DOT, ELISA, IHC, WB |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:20,000 - 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:10,000 - 1:40,000 |
Form/Appearance | Lyophilized |
Purity | Protein G Peroxidase was prepared from chromatographically pure recombinant Protein G. Protein G Peroxidase was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein G. No reaction was observed against anti-Protein A. |
Conjugation | HRP |
Hazard Information | Non-Toxic |
UniProt ID | P19909 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Protein G Peroxidase conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Alternative names | ProG, Streptococcus G protein, Protein G HRP, pero Read more... |
Note | For research use only |
Application notes | Protein G Peroxidase has been tested by ELISA and dot blot and is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110). Protein G Peroxidase is stable at 4° C until restored, at which point it is recommended to freeze aliquots at -20° C. |
Expiration Date | 6 months from date of receipt. |
Human | |
0.16-10 ng/mL | |
0.065 ng/mL |
All | |
3.13-200 ng/mL | |
0.91 ng/mL |
Rat | |
15.63-1000 pg/mL | |
5.7 pg/mL |
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