Protein G Peroxidase Conjugated

SKU: orb348759

Description

Protein G Peroxidase Conjugated

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Application Notes:
  Protein G Peroxidase has been tested by ELISA and dot blot and is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110). 1.54 moles Peroxidase (HRP) per mole. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 1.0 mL

Key Properties

• Purity: Protein G Peroxidase was prepared from chromatographically pure recombinant Protein G. Protein G Peroxidase was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein G. No reaction was observed against anti-Protein A.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Protein G Peroxidase conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Concentration: 1.0 mg/mL
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

ProG, Streptococcus G protein, Protein G HRP, peroxidase, Horseradish peroxidase

Dot Blot of Protein G Peroxidase Conjugated. Antigen 1: Human IgG. Antigen 2: Rat IgG. Antigen 3: Dog IgG. Load: 200 ng followed by a 3-fold serial dilution. Primary antibody: none. Secondary antibody: Protein G Peroxidase Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 60 min at RT.

Quick Database Links

UniProt

P19909