Protein G

• Catalog Number: orb348757
• Category: Proteins
• Description: Protein G
• Tested applications: ELISA, WB
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: User Optimized, WB: User Optimized
• Form/Appearance: Lyophilized
• Purity: Recombinant Protein G is chromatographically pure and shows predominantly a single band by SDS-PAGE. Protein G was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Protein G. No reaction was observed against anti-Protein A.
• Conjugation: Unconjugated
• Hazard Information: Non-Toxic
• UniProt ID: P19909
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Protein G is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: ProG, Streptococcus G protein
• Note: For research use only
• Application notes: Protein G is suitable for use as an antigen, as a control or standard in assays, for conjugation and for most other immunological methods. Protein G contains only IgG binding domains, ensuring maximum specific IgG binding.
• Expiration Date: 6 months from date of receipt.

Dot Blot of Protein G. Antigen 1: Human IgG (p/n orb346219). Antigen 2: Rat IgG. Antigen 3: Dog IgG. Load: Starting dilution is 200 ng followed by a 3-fold serial dilution. Primary antibody: Protein G at 1:1000 for 60 min RT. Secondary antibody: Fluorescein rabbit anti-Protein G (p/n orb344716) at 1:1000 for 30 min at RT. Block: orb348637 for 30 min at RT.