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Protein G Fluorescein Conjugated
SKU: orb348758
Description
Images & Validation
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| Application Notes |
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Key Properties
−| Purity | Protein G Fluorescein Conjugate was prepared from chromatographically pure recombinant Protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Protein G. No reaction was observed against anti-Protein A. |
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| Conjugation | FITC |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
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| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 2.0 mg/mL |
| Expiration Date | 6 months from date of receipt. |
| Hazard Information | Non-Toxic |
| Disclaimer | For research use only |
Alternative Names
−ProG, Streptococcus G protein, Protein G FITC
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Llama IgG Fluorescein Antibody [orb346448]
FC, FLISA, IF, WB
FITC
Llama IgG has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced unconjugated llama IgG1 shows a band of ~150 kDa whereas the reduced form exhibits ~55 kDa (heavy chain) and ~25 kDa (light chain); non-reduced unconjugated llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa; non-reduced unconjugated llama IgG3 shows a band of ~100 kDa whereas the reduced form exhibits ~43 kDa. Fluorescein Conjugated Llama IgG Whole molecule was proven by dot blot.
Llama
100 μg
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Dot Blot results of Protein G Fluorescein Conjugate. Dots are Protein G-FITC: (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Primary Antibody: none. Secondary Antibody: none.
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Protein G Fluorescein Conjugated (orb348758)
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