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Protein G Fluorescein Conjugated
Catalog Number: orb348758
Product Properties
| Catalog Number | orb348758 |
|---|---|
| Category | Proteins |
| Description | Protein G Fluorescein Conjugated |
| Conjugation | FITC |
| Form/Appearance | Lyophilized |
| Concentration | 2.0 mg/mL |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Purity | Protein G Fluorescein Conjugate was prepared from chromatographically pure recombinant Protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Protein G. No reaction was observed against anti-Protein A. |
| UniProt ID | P19909 |
| Tested applications | DOT, FC, FLISA, IF |
| Dilution range | FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000 |
| Application notes | Protein G Fluorescein Conjugated has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays. |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Alternative names | ProG, Streptococcus G protein, Protein G FITC |
| Hazard Information | Non-Toxic |
| Note | For research use only |
Images
Dot Blot results of Protein G Fluorescein Conjugate. Dots are Protein G-FITC: (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Primary Antibody: none. Secondary Antibody: none.
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Llama IgG has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced unconjugated llama IgG1 shows a band of ~150 kDa whereas the reduced form exhibits ~55 kDa (heavy chain) and ~25 kDa (light chain); non-reduced unconjugated llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa; non-reduced unconjugated llama IgG3 shows a band of ~100 kDa whereas the reduced form exhibits ~43 kDa. Fluorescein Conjugated Llama IgG Whole molecule was proven by dot blot.
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Protein G Fluorescein Conjugated (orb348758)
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