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Protein G Alkaline Phosphatase Conjugated

SKU: orb348761

Description

Protein G Alkaline Phosphatase Conjugated

Images & Validation

Tested ApplicationsDOT, ELISA, IHC, WB
Application Notes
Protein G Alkaline Phosphatase Conjugated has been tested by ELISA and dot blot and is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader.

Key Properties

PurityProtein G Alkaline Phosphatase Conjugated was prepared from chromatographically pure recombinant Protein G. Protein G Alkaline Phosphatase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Protein G. No reaction was observed against anti-Protein A.
ConjugationAP

Storage & Handling

StorageStore Protein G at 4° C before opening. DO NOT FREEZE. Protein G Alkaline Phosphatase Conjugated is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form/AppearanceLiquid (sterile filtered)
Buffer/Preservatives0.05% (w/v) Sodium Azide
Concentration1.0 mg/mL
Hazard InformationNon-Toxic
DisclaimerFor research use only

Alternative Names

ProG, Streptococcus G protein, ALP, Alk Phos

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UniProt Details

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
DOT
Dot Blot
View Protocol

Protein G Alkaline Phosphatase Conjugated (orb348761)

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500 μg
$ 680.00
DispatchUsually dispatched within 5-10 working days
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