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Protein G Alkaline Phosphatase Conjugated
Catalog Number: orb348761
| Catalog Number | orb348761 |
|---|---|
| Category | Proteins |
| Description | Protein G Alkaline Phosphatase Conjugated |
| Conjugation | AP |
| Form/Appearance | Liquid (sterile filtered) |
| Concentration | 1.0 mg/mL |
| Buffer/Preservatives | 0.05% (w/v) Sodium Azide |
| Purity | Protein G Alkaline Phosphatase Conjugated was prepared from chromatographically pure recombinant Protein G. Protein G Alkaline Phosphatase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Protein G. No reaction was observed against anti-Protein A. |
| UniProt ID | P19909 |
| Tested applications | DOT, ELISA, IHC, WB |
| Dilution range | ELISA: 1:8,000 - 1:32,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500 |
| Application notes | Protein G Alkaline Phosphatase Conjugated has been tested by ELISA and dot blot and is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader. |
| Storage | Store Protein G at 4° C before opening. DO NOT FREEZE. Protein G Alkaline Phosphatase Conjugated is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
| Alternative names | ProG, Streptococcus G protein, ALP, Alk Phos |
| Hazard Information | Non-Toxic |
| Note | For research use only |
