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Catalog Number | orb427892 |
---|---|
Category | Proteins |
Description | Recombinant of Protein-A/G Cys protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 96.0% as determined by: (a) Analysis by HPLC.(b) Analysis by SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Protein-A/G in sterile 18M-cm H2O not less than 0.1mg/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEEDSLEG SGSGTYKLIL NGKTLKGETT TEAVDAATAE KVFKQYANDN GVDGEWTYDD ATKTFTVTEK PEVIDASELT PAVTTYKLVI NGKTLKGETT TKAVDAETAE KAFKQYANDN GVDGVWTYDD ATKTFTVTEC |
Source | Escherichia Coli |
Storage | Stability: Lyophilized Protein-A/G although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Protein-A/G should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
Buffer/Preservatives | Protein-A/G was lyophilized without any additives. |
Note | For research use only |
Application notes | Recombinant & Natural Proteins |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE | |
Unconjugated | |
> 96% by SDS-PAGE and HPLC analyses. | |
47.8 kDa | |
E. coli |
Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE. | |
Escherichia Coli |
Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE. | |
Escherichia Coli |
SDS-PAGE | |
Unconjugated | |
> 96% by SDS-PAGE and RP-HPLC analyses. | |
47.8 kDa | |
E. coli |
SDS-PAGE | |
Unconjugated | |
> 95% by SDS-PAGE and SEC-HPLC analyses. | |
89.3 kDa | |
E. coli |