Protein A Peroxidase Conjugated

SKU: orb348750

Description

Protein A Peroxidase Conjugated

Images & Validation

• Application Notes:
  Protein A Peroxidase Conjugate has been tested by dot blot and is a useful reagent in Western Blotting and ELISA experiments. Protein A Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

Key Properties

• Purity: Protein A Peroxidase was prepared from chromatographically pure Protein A. Protein A Peroxidase was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein A. No reaction was observed against anti-Protein G.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Protein A Peroxidase conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 6 months from date of receipt.
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

HRP, peroxidase, Horseradish peroxidase protein A

Diagnostic accuracy of detecting IgG and IgG/M antibodies against natural octyl disaccharide-leprosy IDRI diagnostic (NDO-LID) using HRP anti-human IgG (p/n orb347230), HRP anti-human IgM (p/n orb347297), and HRP Protein A. ELISA = enzyme-linked immunosorbent assay; NPV = negative predictive value; PPV = positive predictive value. Results are shown as percent, with confidence intervals in parentheses.

Dot Blot of PEROXIDASE Conjugated Protein A. Lane 1: Human IgG. Lane 2: Golden Syrian Hamster IgG. Lane 3: Sheep IgG. Load: 3-fold serial dilution starting at 200 ng. Primary Antibody: none. Secondary antibody: PEROXIDASE Conjugated Protein A at 1:1000 for 60 min at RT. Block: 1% BSA-TTBS 30 min at RT.

Quick Database Links

UniProt

P38507