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Protein A Peroxidase Conjugated

SKU: orb348749

Description

Protein A Peroxidase Conjugated

Images & Validation

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Application Notes
Protein A Peroxidase Conjugate has been tested by dot blot and is a useful reagent in Western Blotting and ELISA experiments. Protein A Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

Key Properties

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PurityProtein A Peroxidase Conjugation was prepared from chromatographically pure Protein A. Protein A Peroxidase Conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein A. No reaction was observed against anti-Protein G.
ConjugationHRP

Storage & Handling

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StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Protein A Peroxidase Conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/PreservativesPreservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration2.0 mg/mL
Expiration Date6 months from date of receipt.
Hazard InformationNon-Toxic
DisclaimerFor research use only

Alternative Names

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HRP conjugated protein A, peroxidase conjugated Protein A, Horseradish peroxidase conjugated protein A

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UniProt Details

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No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
DOT
Dot Blot
View Protocol

Available Sizes

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2 mg
$ 510.00
DispatchUsually dispatched within 5-10 working days
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