Protein A

SKU: orb348747

Description

Protein A

Images & Validation

• Tested Applications: ELISA, WB
• Application Notes:
  Protein A is suitable for use as an antigen, as a control or standard in assays, for conjugation and for most other immunological methods. Protein A contains only IgG binding domains, ensuring maximum specific IgG binding.

Key Properties

• Purity: Protein A is chromatographically pure and shows predominantly a single band by SDS-PAGE. Greater than 95% of the A280 material binds to Human IgG.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Protein A is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

ProA, Staphylococcus A protein

Impact of behavioral variables on M. leprae infection levels. Anti-natural octyl disaccharide-leprosy IDRI diagnostic (NDO-LID) antibody levels in children and adolescents were measured by ELISA, using either protein A, anti- IgM or anti-IgG to detect responses. In a, samples were stratified by recorded knowledge of eating armadillo meat as either yes (n = 14) or no (n = 64). In b, samples were stratified by recorded knowledge of BCG re-vaccination following identification of the index leprosy case as either yes (n = 54) or no (n = 16). Data are displayed as box and whisker plots, with the box representing the Q1 to Q3 interquartile range and the horizontal bar representing the median of the optical density of the samples. Individual dots indicate outliers, and p-values are indicated by the lines above each indicated group.

Quick Database Links

UniProt

P38507