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Protein A Biotin Conjugated

SKU: orb348752

Description

Protein A Biotin Conjugated

Images & Validation

Application Notes
Protein A Biotin Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype.

Key Properties

PurityThis product was prepared from chromatographically pure Protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Protein A.
ConjugationBiotin

Storage & Handling

StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration1.0 mg/mL
Expiration Date6 months from date of receipt.
Hazard InformationNon-Toxic
DisclaimerFor research use only

Alternative Names

ProA, Staphylococcus A protein, Protein A Biotin Conjugated

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Protein A Biotin Conjugated

Signal response of Nobuto serum samples from 112 canids profiled with the F1-LPA. (A) Detection of F1 immunoreactivity was accomplished using a genus-specific secondary anti-IgG antibody. (B) Detection of F1 immunoreactivity was accomplished using biotinylated conjugates of staphylococcal protein A (p/n orb348752), streptococcal protein G (p/n orb348762), and staphylococcal protein A and streptococcal protein G (A/G), with sera diluted 1:20. The dashed line indicates the threshold for positive and negative samples using an S/N ratio of 10.

UniProt Details

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Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC
Immunohistochemistry
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Protein A Biotin Conjugated (orb348752)

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500 μg
$ 240.00
DispatchUsually dispatched within 5-10 working days
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