Protein A Alkaline Phosphatase Conjugated

• Catalog Number: orb348751
• Category: Proteins
• Description: Protein A Alkaline Phosphatase Conjugated
• Tested applications: DOT, ELISA, IHC, WB
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:8,000 - 1:32,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: Protein A Alkaline Phosphatase Conjugated was prepared from chromatographically pure Protein A. Protein A Alkaline Phosphatase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Protein A. No reaction was observed against anti-Protein G.
• Conjugation: AP
• Hazard Information: Non-Toxic
• UniProt ID: P38507
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. Protein A Alkaline Phosphatase Conjugated is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Alternative names: ProA, Staphylococcus A protein, ALP, Protein A Alk
• Note: For research use only
• Application notes: Protein A Alkaline Phosphatase Conjugated has been tested by ELISA and dot blot and is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader.
• Expiration Date: 6 months from date of receipt.