Protein A Alkaline Phosphatase Conjugated

• Catalog Number: orb348751
• Category: Proteins
• Description: Protein A Alkaline Phosphatase Conjugated
• Conjugation: AP
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Purity: Protein A Alkaline Phosphatase Conjugated was prepared from chromatographically pure Protein A. Protein A Alkaline Phosphatase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Protein A. No reaction was observed against anti-Protein G.
• UniProt ID: P38507
• Tested applications: DOT, ELISA, IHC, WB
• Dilution range: ELISA: 1:8,000 - 1:32,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application notes: Protein A Alkaline Phosphatase Conjugated has been tested by ELISA and dot blot and is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader.
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. Protein A Alkaline Phosphatase Conjugated is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Alternative names: ProA, Staphylococcus A protein, ALP, Protein A Alk
• Hazard Information: Non-Toxic
• Note: For research use only

Antibody responses to multiple Mycobacterium tuberculosis-complex antigens in a meerkat with Mycobacterium bovis infection revealed by multiantigen print immunoassay (MAPIA) of sequential serum samples. Incubated with alkaline phosphatase-conjugated protein A (p/n orb348751). Antigens are indicated in the right margin, with a band visible on each strip where antibody is present. Samples were collected at the following time points: 1 = 0 months (December 2005); 2 = 8 months (August 2006); 3 = 10 months (October 2006); 4 = 15 months (March 2007); 5 = 17 months (May 2007). N = negative control.

Rapid test (lateral flow immunoassay) results of sequential serum samples from a meerkat with Mycobacterium bovis infection. In the test window, the upper line (labeled C) is an internal positive control indicating mobility of the serum sample and diluent across the membrane. The lower bands of reactivity (labeled T) visualized on samples 1–5 indicate a positive reaction to the test antigens. Relative test band strengths are given in brackets: 1 = 0 months (+); 2 = 8 months (++); 3 = 10 months (+++); 4 = 15 months (++); 5 = 17 months (+++). N = negative control. Tracheal washes collected from the same animal at time points 1 and 5 cultured positive for M. bovis.