PRLR Antibody

SKU: orb1251896

Description

PRLR Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC
• Reactivity: Human
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the anti-Prolactin Receptor antibody for each application should be determined by the researcher.1. Staining of formalin/paraffin tissues requires digestion of tissue sections with Pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37oC.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: SPM213
• Immunogen: Semi-purified human prolactin receptor was used as the immunogen for this anti-Prolactin Receptor antibody.
• Target: PRLR
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PRLR, HPRL receptor, PRL-R, HPRLrI, Prolactin receptor

Formalin-fixed, paraffin-embedded human testicular carcinoma stained with anti-Prolactin Receptor antibody (SPM213).

Quick Database Links

Gene Symbol

PRLR

UniProt

P16471