Prolactin Antibody / PRL

• Catalog Number: orb2643005
• Category: Antibodies
• Description: Prolactin is a growth factor that is secreted by the anterior pituitary. It is necessary for the proliferation and differentiation of the mammary glands. Prolactin is useful in the classification of pituitary tumors and study of pituitary disease. It also plays a role in the development of mammary cancer, functioning dually as a mitogen and a differentiating agent.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PRL/2641
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant human partial protein corresponding to amino acids 63-201 was used as the immunogen for the Prolactin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Prolactin antibody is available for research use only.
• UniProt ID: P01236
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human pituitary tissue with Prolactin antibody (clone PRL/2641). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Prolactin antibody (clone PRL/2641). These results demonstrate the foremost specificity of the PRL/2641 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Prolactin antibody (clone PRL/2641) as confirmation of integrity and purity.

Western blot testing of human U937 cell lysate with Prolactin antibody (clone PRL/2641). Predicted molecular weight: ~25 kDa.