PROK1 Antibody (Biotin)

• Catalog Number: orb1272566
• Category: Antibodies
• Description: PROK1 Antibody (Biotin)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hEG-VEGF (Human EG-VEGF).
• Concentration: batch dependent
• Dilution range: ELISA:Sandwich: To detect hEG-VEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human EG-VEGF (XP-5121) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEG-VEGF. Western Blot:To detect hEG-VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEG-VEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Biotin
• Target: PROK1
• UniProt ID: P58294
• NCBI: P58294
• Storage: EG-VEGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: PK1, PRK1, EGVEGFProkineticin-1, Endocrine-gland-d
• Note: For research use only
• Application notes: ELISA:Sandwich: To detect hEG-VEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human EG-VEGF (XP-5121) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEG-VEGF. Western Blot:To detect hEG-VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEG-VEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect hEG-VEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Human EG-VEGF (orb1272567) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hEG-VEGF.