Progesterone Receptor Antibody

• Catalog Number: orb385801
• Category: Antibodies
• Description: Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa). Expression of progesterone receptor has been suggested to reflect a intact estrogen regulatory machinery and therefore predict better clinical response to endocrine therapy than ER alone.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant human Progesterone Receptor protein was used as the immunogen for this antibody.
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Progesterone Receptor antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: PGSR17
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Progesterone Receptor antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE human breast carcinoma with Progesterone Receptor antibody (clone PGSR17). Required HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.