Profilin 1/PFN1 Antibody

• Catalog Number: orb234359
• Category: Antibodies
• Description: Profilin 1/PFN1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Profilin 1 (116-140aa KEGVHGGLINKKCYEMASHLRRSQY), identical to the related mouse and rat sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 15054 MW
• UniProt ID: P07737
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Profilin-1;Epididymis tissue protein Li 184a;Profi
• Note: For research use only
• Application notes: WB: The detection limit for Profilin 1 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of PC-3 cells using anti-Profilin1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Profilin1 using anti-Profilin1 antibody.Lane 1:human Jurkat cell;2:human K562 cell;3:human HeLa cell;4:human MCF-7 cell;5:rat testis tissue;6:rat brain tissue;7:mouse testis tissue;8:mouse brain tissue.

IF analysis of Profilin 1 using anti-Profilin 1 antibody. Profilin 1 was detected in immunocytochemical section of U20S cells.

IHC analysis of Profilin 1 using anti-Profilin 1 antibody. Profilin 1 was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of Profilin 1 using anti-Profilin 1 antibody. Profilin 1 was detected in paraffin-embedded section of human lung cancer tissue.