Sulfanilamide

SKU: orb1310915

Description

Sulfanilamide (UK-124) is a competitive inhibitor of the bacterial enzyme dihydropteroate synthetase, exhibiting an IC50 of 320 µM. This classic antibacterial agent is widely used in biochemical research to study folate biosynthesis inhibition in both in vitro enzymatic assays and in vivo bacterial growth studies.

Research Area

Cell Biology, Infectious Disease & Virology, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 63-74-1
• MW: 172.205
• Purity: 99.94% (May vary between batches)
• Formula: C₆H₈N₂O₂S
• SMILES: NC1=CC=C(C=C1)S(N)(=O)=O
• Target: Antibiotic,Antibacterial,Autophagy
• Solubility: H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:14 mg/mL (81.3 mM);DMSO:50 mg/mL (290.36 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (11.61 mM)

Bioactivity

• Target IC50:
  DHPS:320 μM
• In Vivo:
  Sulfanilamide was able to inhibit a recombinant yeast strain with a wild-type FOL1 gene with an IC50 of 286.8 μg/mL, but a single mutation in the active region of the fungal dihydropyrimidine synthase from 55Trp to 55Ala or 57Pro to 57Ser resulted in resistance to Sulfanilamide with an IC50 >800 μg/mL. /The sulfonamide group of Sulfanilamide inhibits dihydropyridine synthase purified from E. coli. p-Aminobenzoic acid synthesis is used for essential folic acid in the synthesis of purines, pyrimidines, and other amino acids. The IC50 of Sulfanilamide was 320 μM for dihydropyridine synthase, and the Km was 2.5 uM for PABA. Sulfanilamide was able to inhibit the growth of Plasmodium falciparum-containing pKOS-pfPPPK-DHPS(His) bacterial cells to a certain extent with an IC50 of 380 uM.
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• In Vitro:
  Sulfanilamide was able to inhibit a recombinant yeast strain with a wild-type FOL1 gene with an IC50 of 286.8 μg/mL, but a single mutation in the active region of the fungal dihydropyrimidine synthase from 55Trp to 55Ala or 57Pro to 57Ser resulted in resistance to Sulfanilamide with an IC50 >800 μg/mL. /The sulfonamide group of Sulfanilamide inhibits dihydropyridine synthase purified from E. coli. p-Aminobenzoic acid synthesis is used for essential folic acid in the synthesis of purines, pyrimidines, and other amino acids. The IC50 of Sulfanilamide was 320 μM for dihydropyridine synthase, and the Km was 2.5 uM for PABA. Sulfanilamide was able to inhibit the growth of Plasmodium falciparum-containing pKOS-pfPPPK-DHPS(His) bacterial cells to a certain extent with an IC50 of 380 uM.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

UK124, UK-124, UK 124, Sulfanilamide, Sulphanilamide, DHPS, Antibiotic, Bacterial, inhibit, Inhibitor

Compound Identifiers

PubChem CID

5333