Spectinomycin dihydrochloride

SKU: orb1225650

Description

Spectinomycin Dihydrochloride is a new parenteral antibiotic prepared from Streptomyces spectabilis.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 21736-83-4
• MW: 405.27
• Purity: >98% (HPLC)
• Formula: C₁₄H₂₆CL₂N₂O₇
• SMILES: C[C@@H]1CC(=O)[C@]2([C@@H](O1)O[C@@H]3[C@H]([C@@H]([C@@H]([C@@H]([C@H]3O2)NC)O)NC)O)O.Cl.Cl
• Target: ribosome
• Solubility: Water: 81 mg/mL (199.86 mM); DMSO: 81 mg/mL warmed (199.86 mM)

Bioactivity

• In Vivo:
  Spectinomycin dihydrochloride (20 mg/kg; i. m. ; 20-100 mg/kg; 9 d) shows the safety in healthy chicks. Spectinomycin dihydrochloride (10 mg/kg; i. v. ; single dose) has the major elimination pathway by renal excretion, approximately 55% is excreted into the urine in unchanged form. Pharmacokinetics of Spectinomycin dihydrochloride in Rat. Animal model: Arbor Acres plus broiler chicks (15-day-old). Dosage: 20 mg/kg, 60 mg/kg, 100 mg/kg. Administration: Intramuscular injection (chest muscles); 9 days. Result: Showed biosecurity of 20 mg/kg by complete blood count, biochemical parameters, histopathological, clinical signs, body weight gain, and feed conversion ratio (FCR). Resulted minor toxicity of 60 mg/kg.
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• In Vitro:
  Spectinomycin dihydrochloride selectively inhibits protein synthesis in cells and in extracts of Escherichia coli: Spectinomycin dihydrochloride (50 μg/mL) inhibits Escherichia coli growth rapidly and reversibly, and suppresses amino acid incorporation immediately. Spectinomycin dihydrochloride (1 μg/mL or 3 μM) inhibits polypeptide synthesis directed either by endogenous messenger RNA or by MS-2 bacteriophage RNA, with maximum inhibition of 70-80% in extracts of Escherichia coli. Spectinomycin dihydrochloride blocks the translocation of peptidyl-tRNAs from A-site to P-site by inhibiting the binding of elongation factor G to the ribosome. Spectinomycin dihydrochloride interacts specifically with the residues G1064 and 01192 in 16S rRNA and potentially makes it inactive. Spectinomycin dihydrochloride exhibits splicing inhibition and dependent on pH changes and Mg2+ concentration, indicating electrostatic interactions with the intron RNA.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

ribosome