Rat Tumor Necrosis Factor Ligand Superfamily, Member 12 (TNFSF12) ELISA Kit

SKU: orb779872

Description

The Rat Tumor Necrosis Factor Ligand Superfamily, Member 12 (TNFSF12) ELISA Kit uses a sandwich enzyme immunoassay to quantify Tumor Necrosis Factor Ligand Superfamily, Member 12 (TNFSF12) in rat serum, plasma, tissue homogenates, cell lysates and other biological fluids. The kit has a detection range of 31.25-2000 pg/mL and a sensitivity of 13.6 pg/mL.

Research Area

Cancer Biology; Immunology & Inflammation; Cell Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNFSF12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNFSF12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TNFSF12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TNFSF12 in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Rat
• Sample Types: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 13.6 pg/mL
• Concentration: 2000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

APO3L ELISA Kit, DR3LG ELISA Kit, TWEAK ELISA Kit, APO3 ligand ELISA Kit, TNF-related weak inducer of apoptosis ELISA Kit

Quick Database Links

UniProt

Q6AYC1