Rat S100 Calcium Binding Protein A9 (S100A9) ELISA Kit

SKU: orb781408

Description

The Rat S100 Calcium Binding Protein A9 (S100A9) ELISA Kit uses a sandwich enzyme immunoassay to quantify S100 Calcium Binding Protein A9 (S100A9) in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.069 ng/mL.

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat S100A9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat S100A9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat S100A9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat S100A9 in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Rat
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.069 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

MRP14 ELISA Kit, MIF ELISA Kit, 60B8AG ELISA Kit, CFAG ELISA Kit, NIF ELISA Kit, CAGB ELISA Kit, CGLB ELISA Kit, L1AG ELISA Kit, P14 ELISA Kit, Calgranulin B ELISA Kit, Migration inhibitory factor-related protein 14 ELISA Kit, Calprotectin L1H subunit ELISA Kit, Leukocyte L1 complex heavy chain ELISA Kit

Quick Database Links

UniProt

P50116