Rat pro interleukin 1 beta (pro-IL1b) Microsample ELISA Kit

SKU: orb2997486

Description

The Rat pro interleukin 1 beta (pro-IL1b) Microsample ELISA Kit is designed to quantify pro interleukin 1 beta (pro-IL1b) Microsample in rat serum, plasma, tissue homogenates, cell lysates and other biological fluids. The kit has a detection range of 62.5-4000 pg/mL and a sensitivity of 16.7 pg/mL.

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat pro-IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat pro-IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat pro-IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat pro-IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.

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Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 62.5-4000 pg/mL
• Sensitivity: 16.7 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

pro-IL1-B; pro-IL1-Beta; pro-IL1F2; pro-IL-1β