Rat Phosphorylated Adenosine Monophosphate Activated Protein Kinase (p-AMPK) ELISA Kit

SKU: orb1496481

Description

The Rat Phosphorylated Adenosine Monophosphate Activated Protein Kinase (p-AMPK) ELISA Kit is designed to quantify Phosphorylated Adenosine Monophosphate Activated Protein Kinase (p-AMPK) in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 3.13-200 ng/mL and a sensitivity of 1.25 ng/mL.

Research Area

Pharmacology & Drug Discovery

Images & Validation

• Application Notes:
  standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat p-AMPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat p-AMPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat p-AMPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat p-AMPK in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 3.13-200 ng/mL
• Sensitivity: 1.25 ng/mL
• Concentration: 200 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

AMPK