Rat Cluster of Differentiation 30 Ligand (CD30L) ELISA Kit

SKU: orb781479

Description

The Rat Cluster of Differentiation 30 Ligand (CD30L) ELISA Kit uses a sandwich enzyme immunoassay to quantify Cluster of Differentiation 30 Ligand (CD30L) in rat serum, plasma, tissue homogenates and other biological fluids. The kit has a detection range of 125-8000 pg/mL and a sensitivity of 48 pg/mL.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 8000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CD30L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CD30L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CD30L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CD30L in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 125-8000 pg/mL
• Sensitivity: 48 pg/mL
• Concentration: 8000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

CD153 ELISA Kit, TNFSF8 ELISA Kit, CD30-L ELISA Kit, Tumor Necrosis Factor Ligand Superfamily Member 8 ELISA Kit

Quick Database Links

UniProt

P32972