Rat Alkaline Sphingomyelinase (Alk-Smase) ELISA Kit

SKU: orb776493

Description

The Rat Alkaline Sphingomyelinase (Alk-Smase) ELISA Kit uses a sandwich enzyme immunoassay to quantify Alkaline Sphingomyelinase (Alk-Smase) in rat tissue homogenates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.046 ng/mL.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Alk-Smase. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Alk-Smase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Alk-Smase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Alk-Smase in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Rat
• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.046 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

ENPP7 ELISA Kit, E-NPP 7 ELISA Kit, NPP-7 ELISA Kit, Ectonucleotide Pyrophosphatase/Phosphodiesterase 7 ELISA Kit, Sphingomyelin Phosphodiesterase ELISA Kit, Intestinal alkaline sphingomyelinase ELISA Kit

Quick Database Links

UniProt

Q5EZ72