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Description
Research Area
Stem Cell & Developmental Biology
Images & Validation
−| Tested Applications | FC, IF, WB |
|---|---|
| Dilution Range | WB: 0.5-1 μg/ml, IHC-P: 1-2 μg/ml for 30 minutes at RT |
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone No. | PU1/2146 |
| Immunogen | Recombinant fragment (around aa 16-170) of human PU.1 protein (Exact sequence is proprietary) |
| Target | SPI1 |
| Source/Expression System | Bioreactor |
| Molecular Weight | 40kDa |
| Purification | Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Transcription Factor spi1; 31kDa Transforming Protein; Hematopoietic Transcription Factor PU.1; SFPI1; SPI1; SPIA; Spleen focus forming virus (SFFV) proviral integration oncogene spi1
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Quick Database Links
Gene Symbol
SPI1
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence













































