Plant Indole 3 Acetic Acid (IAA) ELISA Kit

SKU: orb782547

Description

The Plant Indole 3 Acetic Acid (IAA) ELISA Kit is designed to quantify Plant Indole 3 Acetic Acid (IAA) in plant tissues. The kit has a detection range of 3.13-200 ng/mL and a sensitivity of 0.88 ng/mL.

Research Area

Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Plant IAA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Plant IAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plant IAA in the samples is then determined by comparing the OD of the samples to the standard curve.

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Key Properties

• Reactivity: Plant
• Sample Types: plant tissues
• Assay Type: Competitive
• Assay Time: 2h
• Range: 3.13-200 ng/mL
• Sensitivity: 0.88 ng/mL
• Concentration: 200 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

IA-A ELISA Kit, Indolylacetic Acid ELISA Kit, Indoleacetic Acid ELISA Kit, Heteroauxin ELISA Kit

Quick Database Links

Gene Symbol

IAA

Sohail Mumtaz 1, Rida Javed 2, Juie Nahushkumar Rana 2, Madeeha Iqbal 2, Eun Ha Choi Pulsed high power microwave seeds priming modulates germination, growth, redox homeostasis, and hormonal shifts in barley for improved seedling growth: Unleashing the molecular dynamics Free Radic Biol Med, (2024)

PubMed: 38901500

Applications

ELISA

Reactivity

Plant