Pig Keratin 18 (KRT18) ELISA Kit

SKU: orb781895

Description

The Pig Keratin 18 (KRT18) ELISA Kit uses a sandwich enzyme immunoassay to quantify Keratin 18 (KRT18) in porcine serum, plasma, tissue homogenates, cell lysates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.054 ng/mL.

Research Area

Cancer Biology; Neuroscience

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig KRT18. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig KRT18. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig KRT18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig KRT18 in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Porcine
• Sample Types: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.054 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

CK18 ELISA Kit, CYK18 ELISA Kit, K18 ELISA Kit, Cytokeratin 18 ELISA Kit, Cell proliferation-inducing gene 46 protein ELISA Kit, Keratin, type I cytoskeletal 18 ELISA Kit