Orotic acid

SKU: orb1310330

Description

Orotic acid, also known as Vitamin B13, is a key biosynthetic precursor in the pyrimidine nucleotide pathway. It is utilized in biochemical research to study nucleotide metabolism, cellular proliferation, and related metabolic disorders in both in vitro and in vivo experimental models.

Research Area

Cell Biology, Metabolism Research, Molecular Biology, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 65-86-1
• MW: 156.0963
• Purity: 99.93% (May vary between batches)
• Formula: C₅H₄N₂O₄
• SMILES: OC(=O)C1=CC(=O)NC(=O)N1
• Target: Nucleoside Antimetabolite/Analog,Endogenous Metabolite,Dehydrogenase
• Solubility: 10% DMSO+90% Corn Oil:2.5 mg/mL (16.02 mM);DMSO:51.5 mg/mL (329.92 mM)

Bioactivity

• In Vivo:
  Male Fischer 344 rats (130-150 g) to two-thirds PH in the absence or in the presence of Orotic acid (a 300-mg tablet of Orotic acid methyl ester implanted intraperitoneally at the time of two-thirds PH). treatment with Orotic acid resulted in a near-100% inhibition of RNR induced by two-thirds PH in rat liver, as monitored by enzyme activity and protein level . Feeding 1% Orotic acid with diet decreased the phosphorylation of AMPK and increased the maturation of SREBP-1 and the expression of SREBP-responsive genes in the rat liver. Orotic acid-induced lipid accumulation was also completely inhibited by rapamycin. Mouse hepatocytes and mice were resistant to Orotic acid-induced lipogenesis because of little if any response in AMPK and downstream effectors .
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• In Vitro:
  Orotic acid increases cell proliferation and decreases apoptosis in serum-starved SK-Hep1 hepatocellular carcinoma cells, which may ascribe to the inhibition of AMP-activated protein kinase (AMPK) phosphorylation and thus activation of mammalian target of rapamycin complex 1 (mTORC1) .
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Endogenous Metabolite, EndogenousMetabolite, hepatomegaly, hepatic, NucleosideAntimetabolite, nucleotides, Nucleoside Antimetabolite, Nucleoside Antimetabolite/Analog, Orotic acid, Inhibitor, inhibit, DHOase, Analog, 6-Carboxyuracil, urea, Vitamin B 13, Vitamin B13, Vitamin B-13, pyrimidine, steatosis, RNA

Compound Identifiers

PubChem CID

967