Nuclear Protein Extraction Kit

SKU: orb1147885

Description

Nuclear Protein Extraction Kit enables the stepwise preparation of cytoplasmic and nuclear extracts from mammalian cells or tissues. The process uses a hypotonic buffer for cell swelling and disruption, followed by a high salt buffer to release nuclear proteins.

Images & Validation

• Application Notes:
  Nuclear Protein Extraction Kit enable stepwise separation and preparation of crude cytoplasmic and nuclear extracts from mammalian cultured cells or tissue. This Kit is based on allowing cells to swell with hypotonic buffer. And then the cells are disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer. Non-denatured, active proteins are purified in less than two hours.

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Storage & Handling

• Storage: Stored at-20°C for 12 months,please store refer to protocols.
• Expiration Date: 6 months from date of receipt.
• Hazard Information: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
• Disclaimer: For research use only