Nitroxoline

SKU: orb1310648

Description

Nitroxoline (5-nitroquinolin-8-ol) is a synthetic antibacterial compound primarily used to treat urinary tract infections due to its efficacy against common uropathogens. Its research applications extend to studying biofilm formation and antimicrobial mechanisms in both in vitro and in vivo experimental models.

Research Area

Cell Biology, Infectious Disease & Virology, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 4008-48-4
• MW: 190.1555
• Purity: 99.86% (May vary between batches)
• Formula: C₉H₆N₂O₃
• SMILES: OC1=CC=C(C2=CC=CN=C12)[N+]([O-])=O
• Target: Apoptosis,Autophagy,Antibacterial,Antibiotic
• Solubility: H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:< 1 mg/mL (insoluble or slightly soluble);DMSO:65 mg/mL (341.82 mM)

Bioactivity

• In Vivo:
  Nitroxoline significantly abrogates tumor growth, angiogenesis and metastasis in vivo in LPB fibrosarcoma and MMTV-PyMT breast cancer mouse models.
• In Vitro:
  Nitroxoline significantly reduces extracellular DQ-collagen IV degradation by all evaluated cancer cell lines using spectrofluorimetry. Nitroxoline also markedly decreases tumor cell invasion monitored in real time and reduces the invasive growth of multicellular tumor spheroids, used as a 3D in vitromodel of tumor invasion. Endothelial tube formation is significantly reduced by nitroxoline in an in vitro angiogenesis assay.
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• Cell Research:
  cell suspension are seeded in the wells of an E-plate 16 according to the manufacturer's instructions. After seeding, the CI is monitored every 15 min. After ∼10 h (MCF-10A neoT and MMTV-PyMT), 14 h (U-87 MG) or 24 h (LPB), when the cells are in their log phase of growth, 50 μl of the compound or 0.1% DMSO is added, and the experiment allowed to run for 72 h. Once every 24 h the medium is replaced with fresh medium containing the inhibitor or suitable control to prevent cell death due to medium depletion. Compounds and their concentrations are: nitroxoline (5 μM) and CA-074 (5 μM) for all cell lines other than MCF-10A neoT cell line, where nitroxoline was used at 2.5 μM. All measurements were performed in quadruplicate. (Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Nitroxoline, inhibit, Inhibitor, 8-Hydroxy-5-nitroquinoline, 5-Nitro-8-hydroxyquinoline, 5-Nitro-8-quinolinol, 5-nitroquinolin-8-ol, Autophagy, Bacterial, Antibiotic, antibacterial

Compound Identifiers

PubChem CID

19910