Neomycin sulfate

SKU: orb1310138

Description

Neomycin sulfate is an aminoglycoside antibiotic that inhibits bacterial protein synthesis. It is widely used as a selection agent in molecular biology to identify cells transfected with neomycin resistance genes, applicable in both in vitro cell culture and in vivo animal model studies.

Research Area

Infectious Disease & Virology, Metabolism Research

Key Properties

• CAS Number: 1405-10-3
• MW: 908.87
• Purity: 99.88% (May vary between batches)
• Formula: C₂₃H₄₆N₆O₁₃·3H₂SO₄
• SMILES: OS(O)(=O)=O.OS(O)(=O)=O.OS(O)(=O)=O.NC[C@@H]1O[C@H](O[C@@H]2[C@@H](CO)O[C@@H](O[C@@H]3[C@@H](O)[C@H](N)C[C@H](N)[C@H]3O[C@H]3O[C@H](CN)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O)[C@H](N)[C@@H](O)[C@@H]1O
• Target: Phospholipase,Antibiotic,Antibacterial,ribosome
• Solubility: DMSO:Insoluble;PBS:100 mg/mL (110.03 mM);H2O:460 mg/mL (506.12 mM)

Bioactivity

• Target IC50:
  PC-PLD:65 µM|ATP-induced current:90 μM
• In Vivo:
  METHODS: To assay in vivo activity, Neomycin sulfate (20 mg/mouse) and bacitracin (20 mg/mouse) were administered by gavage to C57BL/10 mice once daily for seven days. RESULTS: The combination of Neomycin and bacitracin decreased intestinal permeability and increased gene expression of ZO-1, JAM-A, and occludin in the ileum and ZO-1, claudin-3, and claudin-4 in the colon. METHODS: To deplete the intestinal microbiota of mice, the antibiotics (ABX) vancomycin (0.5 g/L), ampicillin (1 g/L), Neomycin sulfate (1 g/L), and metronidazole (1 g/L) were administered to mice by drinking water for two weeks. RESULTS: Antibiotics significantly reduced the diversity and composition of the gut microbiota.
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• In Vitro:
  METHODS: BHK-21 cells, VERO cells and FEA cells were treated with Neomycin sulfate (1000-20000 μg/mL) for 24 h. Cell viability was measured by MTT. RESULTS: The viability of BHK-21 cells was significantly reduced by 9000-20000 μg/mL Neomycin treatment. 3000 μg/mL Neomycin treatment significantly reduced the viability of FEA cells. 20000 μg/mL Neomycin treatment did not affect the viability of VERO cells. METHODS: Mouse cochlear hair cells HEI-OC-1 were treated with Neomycin sulfate (2 mM) for 24 h. Apoptosis was detected by Flow Cytometry. RESULTS: 20 μM GM1 significantly reduced Neomycin sulfate-induced cell death in HEI-OC-1 cells.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Framycin Sulfate, Framycin sulfate, Inhibitor, inhibit, Neomycin, Neomycin sulfate, Neomycin Sulfate, Neomycin sulphate, Antibiotic, angiogenin, aminoglycoside, Bacterial, 30S ribosome, synthesis, ribosomal, proliferation, protein, Phospholipase

Compound Identifiers

PubChem CID

24978553