N-Acetyl-D-galactosamine

SKU: orb1301409

Description

N-Acetyl-D-galactosamine

Research Area

Immunology & Inflammation, Metabolism Research

Key Properties

• CAS Number: 14215-68-0
• MW: 221.208
• Purity: 99.92% (May vary between batches)
• Formula: C₈H₁₅NO₆
• SMILES: CC(N[C@H]1[C@@H](O)O[C@H](CO)[C@H](O)[C@@H]1O)=O
• Target: Others,Endogenous Metabolite,Galectin
• Solubility: H2O:125 mg/mL (565.07 mM)

Bioactivity

• In Vitro:
  N-Acetyl-D-galactosamine (GalNAc or D-GalNAc) on the damage induced by SBA on the membrane permeability and tight junction proteins of piglet intestinal epithelium (IPEC-J2) cells. The IPEC-J2 cells were pre-cultured with 0, 0.125 × 10-4 , 0.25 × 10-4 , 0.5 × 10-4 , 1.0 × 10-4 and 2.0 × 10-4 mmol/L GalNAc at different time period (1, 2, 4 and 8 hr) before being exposed to 0.5 mg/ml SBA for 24 hr.Pre-incubation with GalNAc mitigates the mechanical barrier injury as reflected by a significant increase in trans-epithelial electric resistance (TEER) value and a decrease in alkaline phosphatase (ALP) activity in cell culture medium pre-treated with GalNAc before incubation with SBA as both indicate a reduction in cellular membrane permeability. In addition, mRNA levels of the tight junction proteins occludin and claudin-3 were lower in the SBA-treated groups without pre-treatment with GalNAc. The mRNA expression of occludin was reduced by 17.3% and claudin-3 by 42% (p < 0.01). Moreover, the corresponding protein expression levels were lowered by 17.8% and 43.5% (p < 0.05) respectively. However, in the GalNAc pre-treated groups, occludin and claudin-3 mRNAs were reduced by 1.6% (p > 0.05) and 2.7% (p < 0.01), respectively, while the corresponding proteins were reduced by 4.3% and 7.2% (p < 0.05). In conclusion, GalNAc may prevent the effect of SBA on membrane permeability and tight junction proteins on IPEC-J2s.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GalNAc, N Acetyl D galactosamine, NAcetylDgalactosamine, N-Acetyl-D-galactosamine

Compound Identifiers

PubChem CID

84265