Mouse Tumor Necrosis Factor Alpha Induced Protein 2 (TNFaIP2) ELISA Kit

SKU: orb781273

Description

The Mouse Tumor Necrosis Factor Alpha Induced Protein 2 (TNFaIP2) ELISA Kit uses a sandwich enzyme immunoassay to quantify Tumor Necrosis Factor Alpha Induced Protein 2 (TNFaIP2) in mouse serum, plasma, tissue homogenates and other biological fluids. The kit has a detection range of 78.13-5000 pg/mL and a sensitivity of 30 pg/mL.

Research Area

Cancer Biology; Cardiovascular Research; Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TNFaIP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TNFaIP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TNFaIP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TNFaIP2 in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Mouse
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 78.13-5000 pg/mL
• Sensitivity: 30 pg/mL
• Concentration: 5000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

Primary response gene B94 protein ELISA Kit

Quick Database Links

UniProt

Q61333