Mouse Thymosin Beta 4 (Tb4) ELISA Kit

SKU: orb1291362

Description

The Mouse Thymosin Beta 4 (Tb4) ELISA Kit is designed to quantify Thymosin Beta 4 (Tb4) in mouse serum, plasma and other biological fluids. The kit has a detection range of 3.13-200 ng/mL and a sensitivity of 0.91 ng/mL.

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse Tb4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Tb4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Tb4 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Key Properties

• Reactivity: Mouse
• Sample Types: Serum, plasma and other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 3.13-200 ng/mL
• Sensitivity: 0.91 ng/mL
• Concentration: 200 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

TMSB4X, FX, PTMB4, TB4X, TMSB4, Thymosin,Beta 4,X-Linked, TB-500, Seraspenide, Hematopoietic system regulatory peptide

Quick Database Links

Gene Symbol

Tb4

UniProt

P20065