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Mouse IgG1 Lambda isotype Control Antibody

SKU: orb343762

Description

Mouse IgG1 Lambda isotype Control Antibody

Research Area

Protein Biochemistry

Images & Validation

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Tested ApplicationsELISA, FC, SDS-PAGE
Dilution RangeFC: 1:1000-1:5000
Application Notes
Mouse IgG1 lambda isotype control has been tested in SDS-Page and ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Key Properties

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IsotypeIgG1
Clone No.MG1L
PurityMouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE.
ConjugationUnconjugated

Storage & Handling

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StorageStore vial at 4° C prior to opening. Mouse IgG1 Lambda isotype control is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration1.0 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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Mouse immunoglobulin gamma lambda, Mouse IgG1 isotype control, Mouse IgG1 Lambda

Similar Products

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  • Mouse IgG1 lambda isotype control Antibody [orb3152278]

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    Monoclonal

    Unconjugated

    100 μg
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Protocol Information

FC
Flow Cytometry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Available Sizes

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1 mg
$ 470.00
Free Secondary Antibody (20 ul)0/0

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