Mouse CD5 Antigen Like Protein (CD5L) ELISA Kit

SKU: orb781770

Description

The Mouse CD5 Antigen Like Protein (CD5L) ELISA Kit uses a sandwich enzyme immunoassay to quantify CD5 Antigen Like Protein (CD5L) in mouse serum, plasma and other biological fluids. The kit has a detection range of 0.32-20 ng/mL and a sensitivity of 0.135 ng/mL.

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CD5L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CD5L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CD5L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CD5L in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Mouse
• Sample Types: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.135 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

API6 ELISA Kit, CT-2 ELISA Kit, SP-ALPHA ELISA Kit, Spalpha ELISA Kit, Apoptosis Inhibitor 6 ELISA Kit, Scavenger Receptor Cysteine Rich Family ELISA Kit, IgM-associated peptide ELISA Kit

Quick Database Links

UniProt

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