miRNA 1st Strand cDNA Synthesis Premix (Stem-loop)

SKU: orb3229891

Description

This is a reverse transcription kit for miRNA 1st Strand cDNA synthesis by stem-loop method. This product is optimized for the reaction system. 5X miRNA 1st Strand cDNA Synthesis Premix (Stem-loop) contains the buffer and the buffer for the reverse transcription reaction. M-MLVIt is easy to use and can effectively reduce the loss of reagents and experimental errors, and shorten the operation time by adding only the stem-loop primer, RNA template and water. This product is suitable for reverse transcription of miRNA samples such as Total RNA or Small RNA. The cDNA synthesized by this product is suitable for chimeric qPCR analysis and is recommended to be used with SYBR Green. Pro Taq HS Premixed qPCR Kit II (Code No. orb3229846); for high demand of primer dimer elimination, it can be used with SYBR Green Pro Taq HS Premixed qPCR Kit IV (Code No. orb3229858) for superior results.

Images & Validation

• Application Notes:
  ①This product is a premixed product, the reaction solution is very simple to prepare, only need to add the stem-loop primer, RNA template and water can be directly carried out the reaction. ②This product can reverse transcribe samples containing miRNA such as Total RNA or Small RNA. ③ The cDNA obtained by inversion of this product can realize accurate quantification of mature miRNA with high sensitivity and specificity.

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Storage & Handling

• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only