Membrane and Cytoplasmic Protein Extraction kit

SKU: orb1147882

Description

Membrane and Cytoplasmic Protein Extraction Kit separates non-denatured cytoplasmic and membrane proteins from mammalian samples. After cells are broken by homogenization, membrane proteins are recovered through high-speed centrifugation.

Research Area

Cell Biology

Images & Validation

• Application Notes:
  Membrane and Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of non-denatured, active cytoplasmic and membrane protein from mammalian cultured cells or tissues, which can be used directly in a variety of proteomics application. The cells are first broken by homogenization to allow the release of cytosolic and membrane proteins, after which membrane proteins are obtained by high speed centrifugation. Extraction efficiencies and yields will vary depending on cell type as well as the number of times the integral membrane protein spans the lipid bilayer.

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Storage & Handling

• Storage: Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.
• Expiration Date: 6 months from date of receipt.
• Hazard Information: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
• Disclaimer: For research use only