Hmgn1 Antibody

SKU: orb344408

Description

IPO-38 antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:10,000 - 1:50,000, IHC: 10 µg/mL, WB: 1:500- 1:2,000
• Reactivity: Human
• Application Notes:
  Anti-IPO-38 has been tested by immunohistochemistry (frozen and formalin/paraffin) and western blot analysis on the Raji cell line and reacts with a 14 -16 kDa protein. This antibody is suitable for ELISA and immunoprecipitation. The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells (paraformaldehyde fixed, Triton X-100 permeabilized). An increase of 400% is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemid). IPO-38 does not block the binding of the Ki-67 antibody. Tonsillar tissue is typically used as a positive control.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgM
• Clone No.: IPO-38
• Immunogen: A BALB/c mouse was immunized with spleen cells of a human patient with hairy cell leukemia.
• Target: Hmgn1
• Purity: Anti-IPO-38 monoclonal antibody was purified by Protein A chromatography. This antigen was detected after 12h of PHA-induced activation in the early G1 phase but absent in non-stimulated lymphocytes. Cross reactivity is likely to occur with mouse and rat. Reactivity with other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-IPO38 antibody, mouse anti-HMGN1 antibody, mouse anti-HMG14 antibody, Non-histone chromosomal protein HMG-14 antibody, High-mobility group nucleosome-binding domain-containing protein 1 antibody

Quick Database Links

Gene Symbol

Hmgn1

UniProt

P18608

RefSeq (Protein):NP_032277.3