Intracellular GSH Assay Kit

SKU: orb1409914

Description

This whole cell glutathione assay employs a proprietary thiol-sensitive dye, to monitor relative changes in the intracellular concentration of reduced glutathione (GSH). Analyze the fluorescent signal by flow cytometry.

Images & Validation

• Application Notes:
  1. Prepare samples and controls. 2. Dilute 10X Binding Buffer 1:10 WITH DiH2O. 3. Wash cells twice in ice-cold culture medium or PBS and resuspend in ice-cold 1X Binding Buffer at 5 x 105 – 1 x 106 cells/mL. 4. Reconstitute Bovine Annexin V-Fluorescein in 1 mL Annexin Reconstitution Buffer, 1X. 5. Dilute reconstituted Annexin V-Fluorescein 1:5 in PBS. 6. Add diluted Annexin V-Fluorescein to each sample at ~1:10. 7. Dilute PI 1:10 in PBS. 8. Add diluted PI to each sample at ~1:20. 9. Incubate 15 minutes on ice and protected from light. 10. Add 400 µL 1X Binding Buffer to each sample. 11. Analyze with a flow cytometer. Annexin V-Fluorescein excites at 494 nm and emits at 521 nm. PI excites at 536 nm and emits at 617 nm.

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Key Properties

• Sample Types: Cell culture

Storage & Handling

• Storage: -20°C
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only