Human ProstateSpecific Antigen (PSA) EasyStep ELISA Kit

SKU: orb1817398

Description

The Human ProstateSpecific Antigen (PSA) EasyStep ELISA Kit uses a sandwich enzyme immunoassay to quantify ProstateSpecific Antigen (PSA) EasyStep in human serum, plasma. The kit has a detection range of 0.32-20 ng/mL and a sensitivity of 80 pg/mL.

Research Area

Cancer Biology; Immunology & Inflammation

Images & Validation

• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSA, and the Human PSA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PSA. After TMB substrate solution is added, only those wells that contain Human PSA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSA in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma
• Assay Type: Sandwich
• Assay Time: 1.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 80 pg/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KLK3; APS; KLK2A1; hK3; Kallikrein-3; Kallikrein-Related Peptidase 3; Gamma-seminoprotein