Human Prostate Specific membrane Antigen (PSMA) EasyStep ELISA Kit

SKU: orb1950024

Description

The Human Prostate Specific membrane Antigen (PSMA) EasyStep ELISA Kit uses a sandwich enzyme immunoassay to quantify Prostate Specific membrane Antigen (PSMA) EasyStep in human serum, plasma. The kit has a detection range of 0.63-40 ng/mL and a sensitivity of 0.165 ng/mL.

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

• Application Notes:
  standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSMA, and the Human PSMA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PSMA. After TMB substrate solution is added, only those wells that contain Human PSMA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSMA in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma
• Assay Type: Sandwich
• Assay Time: 1.5h
• Range: 0.63-40 ng/mL
• Sensitivity: 0.165 ng/mL
• Concentration: 40 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cell growth inhibiting protein 27; Cell growth-inhibiting gene 27 protein; FGCP; Folate hydrolase (prostate-specific membrane antigen) 1; Folate hydrolase 1

Quick Database Links

UniProt

Q04609