Human Phospholipase A2, Lipoprotein Associated (LpPLA2) EasyStep ELISA Kit

SKU: orb1950020

Description

The Human Phospholipase A2, Lipoprotein Associated (LpPLA2) EasyStep ELISA Kit uses a sandwich enzyme immunoassay to quantify Phospholipase A2, Lipoprotein Associated (LpPLA2) EasyStep in human serum, plasma. The kit has a detection range of 0.78-50 ng/mL and a sensitivity of 0.78 ng/mL.

Research Area

Infectious Disease & Virology, Metabolism Research, Protein Biochemistry

Images & Validation

• Application Notes:
  standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LpPLA2, and the Human LpPLA2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human LpPLA2. After TMB substrate solution is added, only those wells that contain Human LpPLA2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LpPLA2 in the samples is then determined by comparing the OD of the samples to the standard curve

  Read more...

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma
• Assay Type: Sandwich
• Assay Time: 1.5h
• Range: 0.78-50 ng/mL
• Sensitivity: 0.78 ng/mL
• Concentration: 50 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PLA2G7; PAF-AH; PAFAH; Lp-PLA2; LDL-PLA2; Platelet Activating Factor Acetylhydrolase,Plasma; Phospholipase A2,Group VII; LDL-associated phospholipase A2